rabbit anti e cadherin polyclonal antibody Search Results


e cadherin polyclonal antibody  (Bioss)
94
Bioss e cadherin polyclonal antibody
E Cadherin Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e cadherin polyclonal antibody/product/Bioss
Average 94 stars, based on 1 article reviews
e cadherin polyclonal antibody - by Bioz Stars, 2026-03
94/100 stars
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anti-e-cadherin rabbit polyclonal antibody  (GeneTex)
90
GeneTex anti-e-cadherin rabbit polyclonal antibody
Fabrication of alveolar epithelial cell (AEC) sheets. (a) AECs were subcultured on 35-mm-diameter temperature-responsive culture dishes in alveolar epithelial cell medium (AEpiCM) containing a rho kinase (ROCK) inhibitor (10 mM Y-27632). However, after 7 days of subculture, confluent AECs could not be harvested as a cell sheet when the temperature was reduced from 37 °C to 20 °C for 30 min. Immunohistochemical analysis revealed that cells cultured under these conditions did not express <t>E-cadherin.</t> (b) AECs were subcultured on 35-mm-diameter temperature-responsive culture dishes in AEpiCM containing a ROCK inhibitor, and the cells were confirmed to be confluent after 7 days. Then, the AECs were cultured for a further 3 days in AEpiCM containing a ROCK inhibitor and supplemented with 200 mg/L calcium chloride. Under these conditions, the AECs could be harvested as a cell sheet by reducing the temperature from 37 °C to 20 °C for 30 min. Immunohistochemical analysis revealed that cells cultured using this protocol expressed E-cadherin. (c–h) Hematoxylin and eosin (HE) staining and immunofluorescence staining of AEC sheets fabricated under the feeder-free conditions described in (b). Positive staining for aquaporin-5 (AQP-5), surfactant protein (SP)-A, SP-C, SP-D and Axin-2 showed that the cultured cells were AECs. Cross-sectional (upper panel) and planar (lower panel) views were obtained for the immunohistochemical analyses.
Anti E Cadherin Rabbit Polyclonal Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-e-cadherin rabbit polyclonal antibody/product/GeneTex
Average 90 stars, based on 1 article reviews
anti-e-cadherin rabbit polyclonal antibody - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
pan cadherin polyclonal antibody  (Bioss)
94
Bioss pan cadherin polyclonal antibody
Fabrication of alveolar epithelial cell (AEC) sheets. (a) AECs were subcultured on 35-mm-diameter temperature-responsive culture dishes in alveolar epithelial cell medium (AEpiCM) containing a rho kinase (ROCK) inhibitor (10 mM Y-27632). However, after 7 days of subculture, confluent AECs could not be harvested as a cell sheet when the temperature was reduced from 37 °C to 20 °C for 30 min. Immunohistochemical analysis revealed that cells cultured under these conditions did not express <t>E-cadherin.</t> (b) AECs were subcultured on 35-mm-diameter temperature-responsive culture dishes in AEpiCM containing a ROCK inhibitor, and the cells were confirmed to be confluent after 7 days. Then, the AECs were cultured for a further 3 days in AEpiCM containing a ROCK inhibitor and supplemented with 200 mg/L calcium chloride. Under these conditions, the AECs could be harvested as a cell sheet by reducing the temperature from 37 °C to 20 °C for 30 min. Immunohistochemical analysis revealed that cells cultured using this protocol expressed E-cadherin. (c–h) Hematoxylin and eosin (HE) staining and immunofluorescence staining of AEC sheets fabricated under the feeder-free conditions described in (b). Positive staining for aquaporin-5 (AQP-5), surfactant protein (SP)-A, SP-C, SP-D and Axin-2 showed that the cultured cells were AECs. Cross-sectional (upper panel) and planar (lower panel) views were obtained for the immunohistochemical analyses.
Pan Cadherin Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pan cadherin polyclonal antibody/product/Bioss
Average 94 stars, based on 1 article reviews
pan cadherin polyclonal antibody - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier

Image Search Results


Fabrication of alveolar epithelial cell (AEC) sheets. (a) AECs were subcultured on 35-mm-diameter temperature-responsive culture dishes in alveolar epithelial cell medium (AEpiCM) containing a rho kinase (ROCK) inhibitor (10 mM Y-27632). However, after 7 days of subculture, confluent AECs could not be harvested as a cell sheet when the temperature was reduced from 37 °C to 20 °C for 30 min. Immunohistochemical analysis revealed that cells cultured under these conditions did not express E-cadherin. (b) AECs were subcultured on 35-mm-diameter temperature-responsive culture dishes in AEpiCM containing a ROCK inhibitor, and the cells were confirmed to be confluent after 7 days. Then, the AECs were cultured for a further 3 days in AEpiCM containing a ROCK inhibitor and supplemented with 200 mg/L calcium chloride. Under these conditions, the AECs could be harvested as a cell sheet by reducing the temperature from 37 °C to 20 °C for 30 min. Immunohistochemical analysis revealed that cells cultured using this protocol expressed E-cadherin. (c–h) Hematoxylin and eosin (HE) staining and immunofluorescence staining of AEC sheets fabricated under the feeder-free conditions described in (b). Positive staining for aquaporin-5 (AQP-5), surfactant protein (SP)-A, SP-C, SP-D and Axin-2 showed that the cultured cells were AECs. Cross-sectional (upper panel) and planar (lower panel) views were obtained for the immunohistochemical analyses.

Journal: Regenerative Therapy

Article Title: A novel alveolar epithelial cell sheet fabricated under feeder-free conditions for potential use in pulmonary regenerative therapy

doi: 10.1016/j.reth.2022.01.005

Figure Lengend Snippet: Fabrication of alveolar epithelial cell (AEC) sheets. (a) AECs were subcultured on 35-mm-diameter temperature-responsive culture dishes in alveolar epithelial cell medium (AEpiCM) containing a rho kinase (ROCK) inhibitor (10 mM Y-27632). However, after 7 days of subculture, confluent AECs could not be harvested as a cell sheet when the temperature was reduced from 37 °C to 20 °C for 30 min. Immunohistochemical analysis revealed that cells cultured under these conditions did not express E-cadherin. (b) AECs were subcultured on 35-mm-diameter temperature-responsive culture dishes in AEpiCM containing a ROCK inhibitor, and the cells were confirmed to be confluent after 7 days. Then, the AECs were cultured for a further 3 days in AEpiCM containing a ROCK inhibitor and supplemented with 200 mg/L calcium chloride. Under these conditions, the AECs could be harvested as a cell sheet by reducing the temperature from 37 °C to 20 °C for 30 min. Immunohistochemical analysis revealed that cells cultured using this protocol expressed E-cadherin. (c–h) Hematoxylin and eosin (HE) staining and immunofluorescence staining of AEC sheets fabricated under the feeder-free conditions described in (b). Positive staining for aquaporin-5 (AQP-5), surfactant protein (SP)-A, SP-C, SP-D and Axin-2 showed that the cultured cells were AECs. Cross-sectional (upper panel) and planar (lower panel) views were obtained for the immunohistochemical analyses.

Article Snippet: The sections were treated with anti-SP-A rabbit polyclonal antibody (1:200 dilution; bs-10265R; Bioss), anti-SP-C rabbit polyclonal antibody (1:100 dilution; bs-10067R; Bioss), anti-SP-D rabbit polyclonal antibody (1:200 dilution; bs-1583R; Bioss), anti-AQP-5 rabbit polyclonal antibody (1:200 dilution; bs-1554R; Bioss), anti-Axin-2 rabbit polyclonal antibody (1:100 dilution; GTX31822; Genetex) or anti-E-cadherin rabbit polyclonal antibody (1:100 dilution; GTX477; Genetex) at 4 °C overnight.

Techniques: Immunohistochemical staining, Cell Culture, Staining, Immunofluorescence